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Chemical Sciences

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Molecular Absorption Spectroscopy Lab

  1. Familiarization with the UV-Visible Absorption Spectroscopy:
  2. Solvent Effects on the UV-visible Absorption Spectra
  3. Effects of sample path length Dependence of Absorbance (Lambert Law)
  4. Effects of sample concentration Dependence of Absorbance (Beer Law)
  5. Determination of the Molar Absorptivity of a Light Absorbing Molecule
  6. Verification of Beer-Lambert Law
  7. Determination of unknown concentration of an analyte by using the Beer-Lambert Law
  8. Simultaneous estimation method for multicomponent samples: Principle of additivity of absorbance
  9. pH effects on absorption spectra: pKa determination by spectrophotometric method
  10. Study of kinetics of a reaction by using spectrophotometric method

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